Areas of Interest
noncoding RNA • Multi-omics • Cancer biology
We are interested in how cellular signals are propagated through interaction networks at the RNA-protein interface, and how these networks malfunction during cancer and disease. While much of the human genome is transcribed into RNA, only a small portion (< 5%) encodes protein; the remainder of the transcriptome includes various types of small and long noncoding RNAs whose mechanisms are not fully understood. Some long noncoding RNAs associate with chromatin in the nucleus as a part of larger networked ribonucleoprotein granules, where they regulate gene expression and RNA processing. In cancer cells, changes in RNA-binding protein stoichiometry and modification status reorganize the interaction networks of noncoding RNAs, resulting in altered functions. Our vision is to leverage RNA and protein sequencing technologies to characterize these altered interaction network profiles in cancer cells and generate novel therapeutic strategies that target the RNA-protein interface.
Honors & Awards
Biological Sciences Scholar, University of Michigan Medical School, 2021
American Cancer Society Postdoctoral Fellowship, American Cancer Society, 2017–2020
Rackham Predoctoral Fellowship, University of Michigan, 2014
Minor J. and Mary Lou Coon Award, University of Michigan Medical School, 2014
Single-Molecule Correlated Chemical Probing: A Revolution in RNA Structure Analysis.
Mustoe AM, Weidmann CA, Weeks KM.
Acc Chem Res. 2023; 56: 763–75.
Structural Analysis of MALAT1 long non-coding RNA in cells and in evolution.
Monroy-Eklund A, Taylor C, Weidmann CA, Burch C, Laederach A.
RNA. 2023; 29: 691–704.
Global 5'-UTR RNA structure regulates translation of a SERPINA1 mRNA.
Grayeski PJ, Weidmann CA, Kumar J, Lackey L, Mustoe AM, Busan S, Laederach A, Weeks KM.
Nucleic Acids Res. 2022; 50: 9689–704.
Discovery of a large-scale, cell-state-responsive allosteric switch in the 7SK RNA using DANCE-MaP.
Olson SW, Turner AW, Arney JW, Saleem I, Weidmann CA, Margolis DM, Weeks KM, Mustoe AM.
Mol Cell. 2022; 82: 1708–23.
Distinct MUNC lncRNA structural domains regulate transcription of different promyogenic factors.
Przanowska RK, Weidmann CA, Saha S, Cichewicz MA, Jensen KN, Przanowski P, Irving PS, Janes KA, Guertin MJ, Weeks KM, Dutta A.
Cell Rep. 2022; 38: 110361.
Analysis of RNA-protein networks with RNP-MaP defines functional hubs on RNA.
Weidmann CA, Mustoe AM, Jariwala PB, Calabrese JM, Weeks KM.
Nat Biotechnol. 2021; 39: 347–56.
Genomic RNA Elements Drive Phase Separation of the SARS-CoV-2 Nucleocapsid.
Iserman C*, Roden CA*, Boerneke MA, Sealfon RSG, McLaughlin GA, Jungreis I, Fritch EJ, Hou YJ, Ekena J, Weidmann CA, Theesfeld CL, Kellis M, Troyanskaya OG, Baric RS, Sheahan TP, Weeks KM, Gladfelter AS.
Mol Cell. 2020; 80: 1078–91.
Targeting the Oncogenic Long Non-coding RNA SLNCR1 by Blocking Its Sequence-Specific Binding to the Androgen Receptor.
Schmidt K*, Weidmann CA*, Hilimire TA, Yee E, Hatfield BM, Schneekloth JS Jr, Weeks KM, Novina CD.
Cell Rep. 2020; 30: 541–54.
SHAPE Probing Reveals Human rRNAs Are Largely Unfolded in Solution.
Giannetti CA, Busan S, Weidmann CA, Weeks KM.
Biochemistry. 2019; 58: 3377–85.
Guidelines for SHAPE Reagent Choice and Detection Strategy for RNA Structure Probing Studies.
Busan S*, Weidmann CA*, Sengupta A, Weeks KM.
Biochemistry. 2019; 58: 2655–64.
mRNA structure determines specificity of a polyQ-driven phase separation.
Langdon EM, Qiu Y, Ghanbari Niaki A, McLaughlin GA, Weidmann CA, Gerbich TM, Smith JA, Crutchley JM, Termini CM, Weeks KM, Myong S, Gladfelter AS.
Science. 2018; 360: 922–27.
For a list of publications from MyNCBI, click HERE