Areas of Interest
The DNA of eukaryotes is packaged into chromatin by the wrapping of ~146 bp of DNA around a core histone octamer. This packaging serves to compact the DNA into the nucleus, but also presents a barrier to cellular processes, such as transcription, replication and repair, which require access to the DNA. Therefore, controlling access to the chromosomal DNA represents an important regulatory point for these processes. Two major classes of enzymes control access to chromatin. The first class includes enzymes that covalently modify the histones. Examples include acetyltransferases and deacetylases, methyltransferases and demethylases, kinases, and ubiquitin ligases. The second major class of enzymes uses the energy of ATP to alter the structure and/or location of the nucleosome. The targeting of these factors to chromatin determines the transcriptional output of a given gene. Control of these transcriptional events can subsequently coordinate cellular events such as apoptosis, proliferation, and differentiation. Disruption of these events is critical to many pathologies including neoplastic transformation and developmental disorders. Indeed, several genes encoding components of chromatin regulatory complexes have been implicated as etiologic agents in many cancers and hereditary malformations.
Honors & Awards
New Investigator Award, Prostate Cancer Research Program, Department of the Army, 2007–2010
Research Scholar Grant, American Cancer Society, 2007–2011
Biological Sciences Scholar, University of Michigan Medical School, 2003
Christopher Davis Memorial Fellowship for Breast Cancer, 2001–2002
Ching Jer Chern Memorial Award, Best Scientific Publication, Wistar Institute, 1999
Hyperphosphorylated tau aggregation and cytotoxicity modulators screen identified prescription drugs linked to Alzheimer's disease and cognitive functions.
Liu M, Dexheimer T, Sui D, Hovde S, Deng X, Kwok R, Bochar DA, Kuo MH.
Sci Rep. 2020; 10: 16551.
Hyperphosphorylation Renders Tau Prone to Aggregate and to Cause Cell Death.
Liu M, Sui D, Dexheimer T, Hovde S, Deng X, Wang KW, Lin HL, Chien HT, Kweon HK, Kuo NS, Ayoub CA, Jimenez-Harrison D, Andrews PC, Kwok R, Bochar DA, Kuret J, Fortin J, Tsay YG, Kuo MH.
Mol Neurobiol. 2020; 57: 4704–19.
Regulation of androgen-responsive transcription by the chromatin remodeling factor CHD8.
Menon T, Yates JA, Bochar DA.
Mol Endocrinol. 2010; 24: 1165–74.
Regulation of HOXA2 gene expression by the ATP-dependent chromatin remodeling enzyme CHD8.
Yates JA, Menon T, Thompson BA, Bochar DA.
FEBS Lett. 2010; 584: 689–93.
Research into selective biomarkers of erythrocyte exposure to organophosphorus compounds.
Aminoff D, Bochar DA, Fuller AA, Mapp AK, Showalter HD, Kirchhoff PD.
Anal Biochem. 2009; 392: 155–61.
CHD8 is an ATP-dependent chromatin remodeling factor that regulates beta-catenin target genes.
Thompson BA, Tremblay V, Lin G, Bochar DA.
Mol Cell Biol. 2008; 28: 3894–904.
Regulation of tryptophan hydroxylase-2 gene expression by a bipartite RE-1 silencer of transcription/neuron restrictive silencing factor (REST/NRSF) binding motif.
Patel PD, Bochar DA, Turner DL, Meng F, Mueller HM, Pontrello CG.
J Biol Chem. 2007; 282: 26717–24.
For a list of publications from PubMed, click HERE