After obtaining a B.Sc. in Biochemistry from the University of Leeds in England, Georgios Skiniotis moved to Germany to pursue his Ph.D. in the group of Dr. Andreas Hoenger at the European Molecular Biology Laboratory in Heidelberg. At EMBL, while receiving further training in biochemistry and molecular biology, he specialized in electron cryomicroscopy and helical reconstructions to study the interaction of kinesin motors with microtubules. Resulting in numerous publications, his structural and biochemical work on kinesin-microtubule complexes provided significant new insights to the "walking" mechanism of molecular motors.
Following the completion of his Ph.D., and with an ever-growing fascination for the visualization of protein complexes by electron microscopy, Dr. Skiniotis crossed the Atlantic to join the lab Dr. Thomas Walz at Harvard Medical School in Boston. In Harvard, where he was awarded with the prestigious Damon Runyon fellowship, he strengthened his electron microscopy background with the application of single particle analysis techniques for the structural investigation of macromolecular complexes. Besides his work on structural studies of RSC, a 15-subunit chromatin remodeling machine in yeast, he also engaged in collaborations for the structural characterization of several signaling cell surface receptors, such as gp130, LIF-R, and the Vascular Endothelial Growth Factor Receptor (VEGF-R).
Dr. Skiniotis joined LSI as a Biological Sciences Scholar and Research Assistant Professor in 2008.
Areas of Interest
His primary research focus is the application of molecular electron microscopy for the exploration of chromatin modifying complexes and the mechanisms involved in nucleosome remodeling. In addition, he continues working on cell surface receptors and the ways their extracellular architectures instigate intracellular signaling.
Shukla AK, Westfield GH, Xiao K, Reis RI, Huang LY, Tripathi-Shukla P, Qian J, Li S, Blanc A, Oleskie AN, Dosey AM, Su M, Liang CR, Gu LL, Shan JM, Chen X, Hanna R, Choi M, Yao XJ, Klink BU, Kahsai AW, Sidhu SS, Koide S, Penczek PA, Kossiakoff AA, Woods VL Jr, Kobilka BK✚, Skiniotis G✚& Lefkowitz RJ✚. (2014). Visualization of arrestin recruitment by a G Protein-Coupled Receptor. Nature. PubMed link
Dutta S, Whicher JR, Hansen DA, Hale WA, Chemler JA, Congdon GR, Narayan AR, Håkansson K, Sherman DH, Smith JL,Skiniotis G. (2014). Structure of a modular polyketide synthase. Nature. PubMed link
Whicher JR, Dutta S, Hansen DA, Hale WA, Chemler JA, Dosey AM, Narayan AR, Håkansson K, Sherman DH, Smith JL,Skiniotis, G. (2014). Structural rearrangements of a polyketide synthase module during its catalytic cycle. Nature. PubMed link
Akey DL, Brown WC, Dutta S, Konwerski J, Jose J, Jurkiw TJ, DelProposto J, Ogata CM, Skiniotis G, Kuhn RJ, Smith JL. (2014). Flavivirus NS1 structures reveal surfaces for associations with membranes and the immune system. Science. PubMed link
Vashisth H, Skiniotis G, Brooks CL 3rd. (2014) Collective variable approaches for single molecule flexible fitting and enhanced sampling. Chemical Reviews. PubMed link
Thornton JL, Westfield GH, Takahashi YH, Cook M, Gao X, Woodfin AR, Lee JS, Morgan MA, Jackson J, Smith ER, Couture JF, Skiniotis G, Shilatifard A. (2014). Context dependency of Set1/COMPASS-mediated histone H3 Lys4 trimethylation. Genes and Development. PubMed link
Patterson DP, Su M, Franzmann TM, Sciore A, Skiniotis G, Marsh EN. (2014). Characterization of a highly flexible self-assembling protein system designed to form nanocages. Protein Science. PubMed link
Skiniotis G*, Lupardus P*, Martick M, Walz T & Garcia KC. Molecular architecture of a full-length cytokine receptor transmembrane signaling complex: the quaternary assembly of CNTF, CNTF-Ra, LIF-R and gp130. (*: equal contribution) Molecular Cell 2008 Sep 5;31(5):737-48. PubMed Link
Meijers R, Puettmann-Holgado R, Skiniotis G, Liu J-H, Walz T, Schmucker D & Wang J-H. Structural basis of Dscam isoform specificity. Nature 2007 Sep 27; 449 (7161):487-91. PubMed Link
Skiniotis G, Moazed D and Walz T. Acetylated histone-tail peptides induce structural rearrangements in the RSC chromatin remodeling complex. J Biol Chem. 2007 July 20;282(29):20804-8. PubMed Link
Ruch C*, Skiniotis G*, Steinmetz M, Walt T & Ballmer-Hofer K. Structure of a VEGF / VEGF receptor complex as determined by EM. (*: equal contribution) Nature Struct Mol Biol. 2007 Mar; 14(3):249-250. PubMed Link
Skiniotis G, Boulanger MJ, Garcia KC, and Walz T. Signaling conformations of the tall cytokine receptor gp130 when in complex with IL-6 and IL-6 receptor. Nature Struct Mol Biol. 2005 Jun;12(6):545-51. PubMed Link
Santarella RA, Skiniotis G, Goldie KN, Tittmann P, Gross H, Mandelkow EM, Mandelkow E, and Hoenger A. Surface-decoration of microtubules by human tau. J Mol Biol. 2004 Jun 4;339(3):539-53 PubMed Link
Skiniotis G, Cochran JC, Muller J, Gilbert SP, and Hoenger A. Modulation of kinesin binding by the C-termini of tubulin. EMBO J. 2004 Mar 10;23(5):989-99. PubMed Link
Bringmann H, Skiniotis G, Spilker A, Kandels-Lewis S, Vernos I, Surrey T. A kinesin-like motor inhibits microtubule dynamic instability. Science. 2004 Mar 5;303(5663):1519-22. PubMed Link
Skiniotis G, Surrey T, Altmann S, Gross H, Song YH, Mandelkow E, Hoenger A. Nucleotide-induced conformations in the neck region of dimeric kinesin. EMBO J. 2003 Apr 1;22(7):1518-28. PubMed Link
Wendt TG, Volkmann N, Skiniotis G, Goldie KN, Muller J, Mandelkow E, Hoenger A. Microscopic evidence for a minus-end-directed power stroke in the kinesin motor ncd. EMBO J. 2002 Nov 15;21(22):5969-78. PubMed Link
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