Areas of Interest
Intracellular transport * Motor proteins * Structural biology * Single molecule biophysics * Cryo-electron microscopy * Cloud computing
We are a research team that is trying to understand the molecular details that determine how, where, and when motor proteins transport intracellular cargo. The past thirty years of cell biology research have set the stage for us to determine the general principles that underlie the complex process of intracellular transport.
Specifically, we are interested in the mechanisms that dictate how cytoplasmic dynein and kinesin are recruited to—and activated for—cargo transport. For each project, we will be trying to answer the following questions:
- How is specificity determined between motor proteins and cargo?
- How are the activities of multi-motor complexes regulated?
- What are the molecular consequences of neurodegenerative disease-causing mutations?
We approach these questions using state-of-the-art technologies that range from mammalian cell protein expression to cryo-EM to single molecule fluorescence assays. This integrated approach will allow us to relate how changes at the molecular level alter the structure and function of transporting motor-cargo complexes.
Tool development for cryo-electron microscopy
As a fast-growing part of structural biology, cryo-electron microscopy (cryo-EM) is determining new and exciting macromolecular structures on a seemingly daily basis. Despite its power, cryo-EM is a field that needs to undergo rapid maturation to allow for new users to come into the fold to solve structures. Unlike other structural biology tools, cryo-EM necessarily requires access to high-performance computing capabilities. The large computational workload will limit the throughput and spread of cryo-EM due to users 1) waiting for cluster time or 2) being unable to find a cluster amenable for cryo-EM.
To address these problems, we are building cloud computing resources at Amazon Web Services and the San Diego Supercomputer Center to help give users access to cryo-EM so they can focus on understanding biology instead of deal with Linux. In addition to these new software tools, we are also considering new methods that will give un-supervised assessment of single particle electron microscopy data quality, given the large computing resources of the cloud.
Kinesin-1, -2 and -3 motors use family-specific mechanochemical strategies to effectively compete with dynein during bidirectional transport.
Gicking AM, Ma TC, Feng Q, Jiang R, Badieyan S, Cianfrocco MA, Hancock WO.
Elife. 2022; 11: e82228.
A kinesin-1 variant reveals motor-induced microtubule damage in cells.
Budaitis BG, Badieyan S, Yue Y, Blasius TL, Reinemann DN, Lang MJ, Cianfrocco MA, Verhey KJ.
Curr Biol. 2022; 32: 2416–29.
Cloud computing platforms to support cryo-EM structure determination.
Li Y, Cianfrocco MA.
Trends Biochem Sci. 2022; 47: 103–5.
Kinesin-binding protein remodels the kinesin motor to prevent microtubule binding.
Solon AL, Tan Z, Schutt KL, Jepsen L, Haynes SE, Nesvizhskii AI, Sept D, Stumpff J, Ohi R, Cianfrocco MA.
Sci Adv. 2021; 7: eabj9812.
A mitochondrial membrane-bridging machinery mediates signal transduction of intramitochondrial oxidation.
Li L, Conradson DM, Bharat V, Kim MJ, Hsieh CH, Minhas PS, Papakyrikos AM, Durairaj AS, Ludlam A, Andreasson KI, Partridge L, Cianfrocco MA, Wang X.
Nat Metab. 2021; 3: 1242–58.
Molecular determinants for α-tubulin methylation by SETD2.
Kearns S, Mason FM, Rathmell WK, Park IY, Walker C, Verhey KJ, Cianfrocco MA.
J Biol Chem. 2021; 297: 100898.
Parthenolide Destabilizes Microtubules by Covalently Modifying Tubulin.
Hotta T, Haynes SE, Blasius TL, Gebbie M, Eberhardt EL, Sept D, Cianfrocco M, Verhey KJ, Nesvizhskii AI, Ohi R.
Curr Biol. 2021; 31: 900–7.
For a list of publications at Google Scholar, click HERE