Researchers from the Yan Zhang Lab in the Department of Biological Chemistry at the University of Michigan, in collaboration with other Michigan, Cornell, and University of Melbourne scientists, developed a novel "DNA shredder" type of CRISPR-Cas3 technology in human embryonic stem cells, opening doors to long-range genome interrogation and epigenome modification. This is also the first time that any of the prevalent CRISPR systems with their multi-subunit effector complexes being adopted for eukaryotic use.
U-M senior staff scientist Zhonggang Hou, Ph.D. and Cornell graduate student Adam Dolan are the paper’s first authors. Other U-M co-authors include graduate student Max Gramelspacher, undergraduate student Jaewon Heo, and Peter Freddolino, Ph.D., assistant professor of biological chemistry and computational medicine and bioinformatics.
Read the published paper here in Molecular Cell https://www.cell.com/molecular-cell/fulltext/S1097-2765(19)30217-5