Areas of Interest
The regulatory networks of bacteria play a key role in their information processing capabilities, coordinating and executing interactions with their environments. Quantitative, predictive models of these networks would be tremendously beneficial for facilitating the development of new antimicrobial therapies, enabling synthetic biology applications, and understanding bacterial evolution and ecology. Ultimately, the aim of my laboratory is to build a multiscale framework enabling modeling of bacterial regulatory networks at any level of detail, from atomistic to cellular. To this end, we develop and apply high-throughput experimental methods for measuring biomolecular interactions and cellular regulatory states in vivo, and for profiling the phenotypic consequences of regulatory changes. In tandem with these experimental approaches, we use molecular simulation and mathematical modeling to obtain high-resolution insight into the biomolecular interactions driving regulatory networks, and the systems-level effects of altering them.
Detecting Gene Ontology misannotations using taxon-specific rate ratio comparisons.
Wei X, Zhang C, Freddolino PL, Zhang Y.
Bioinformatics. 2020, in press.
A Well-Mixed E. coli Genome: Widespread Contacts Revealed by Tracking Mu Transposition.
Walker DM, Freddolino PL, Harshey RM.
Cell. 2020; 180: 703–16.
Unique repression domains of Pumilio utilize deadenylation and decapping factors to accelerate destruction of target mRNAs.
Arvola RM, Chang CT, Buytendorp JP, Levdansky Y, Valkov E, Freddolino PL, Goldstrohm AC.
Nucleic Acids Res. 2020; 48: 1843–71.
Rapid metabolic shifts occur during the transition between hunger and satiety in Drosophila melanogaster.
Wilinski D, Winzeler J, Duren W, Persons JL, Holme KJ, Mosquera J, Khabiri M, Kinchen JM, Freddolino PL, Karnovsky A, Dus M.
Nat Commun. 2019; 10: 4052.
High-Resolution Mapping of the Escherichia coli Chromosome Reveals Positions of High and Low Transcription.
Scholz SA, Diao R, Wolfe MB, Fivenson EM, Lin XN, Freddolino PL.
Cell Syst. 2019; 8: 212–25.
Global analysis of RNA metabolism using bio-orthogonal labeling coupled with next-generation RNA sequencing.
Wolfe MB, Goldstrohm AC, Freddolino PL.
Methods. 2019; 155: 88–103.
Escherichia coli Lrp Regulates One-Third of the Genome via Direct, Cooperative, and Indirect Routes.
Kroner GM, Wolfe MB, Freddolino PL.
J Bacteriol. 2019; 201: e00411-18.
For a list of publications at Google Scholar, click HERE