Areas of Interest
My current research project is focused on elucidating interactions between human DNA ligase I (LIG1) and its substrate DNA using both structural and kinetic techniques. LIG1 is the main replicative DNA ligase in eukaryotes and is required for normal cell growth. Currently, I am characterizing active site mutants of LIG1 and analyzing the effects of perturbations of its ability to perform specific steps of its catalytic mechanism. I hope to follow this with using X-ray crystallography to gain structural insights into the interactions between LIG1 and a nicked-DNA substrate, which will hopefully enhance our understanding of how LIG1 is able to identify a nicked-DNA substrate for catalysis.
Mentor: Dr. Pat O'Brien
2014 - Thomas Jurkiw, Mark Taylor and Patrick O’Brien; Functional Analysis of Conserved Amino Acids in DNA Ligase I. Presented at the Midwest DNA Repair Symposium, Wayne State University, Detroit, MI, May 17, 2014. Poster Presentation.
Honors & Awards
2014 Cellular Biotechnology Training Program
1. David L. Akey, W. Clay Brown, Somnath Dutta, Jamie Konwerski, Joyce Jose, Thomas J. Jurkiw, James DelProposto, Craig M. Ogata, Georgios Skiniotis, Richard J. Kuhn, and Janet L. Smith (2014). Flavivirus NS1 Structures Reveal Surfaces for Associations with Membranes and the Immune System. Science, 343(6173), 881–885 PMID: 24505133
For a complete list of this person’s PubMed publications, click HERE