Areas of Interest
CRISPR-Cas is a RNA-guided, genetic interference pathway in prokaryotes that enables acquired immunity against invasive nucleic acids. Nowadays, CRISPRs also provide formidable tools for facile, programmable genome engineering in eukaryotes. Cas9 proteins are the “effector” endonucleases for CRISPR interference; and have recently begun to be also recognized as important players in other aspects of bacterial physiology (e.g. acquisition of new spacers into CRISPRs, endogenous gene regulation, and microbial pathogenesis, etc.). My laboratory is broadly interested in CRISPR biology and mechanism. We will use Neisseria species as our model system, and E. coli and human cells as additional platforms. We employ complementary biochemical, microbiological, genetic and genomic approaches. We are also interested in working with the broader scientific community to develop and apply novel CRISPR-based tools to tackle diverse biological questions.
Introducing a Spectrum of Long-Range Genomic Deletions in Human Embryonic Stem Cells Using Type I CRISPR-Cas.
Dolan AE, Hou Z, Xiao Y, Gramelspacher MJ, Heo J, Howden SE, Freddolino PL, Ke A, Zhang Y.
Mol Cell. 2019; in press.
Insights into a Mysterious CRISPR Adaptation Factor, Cas4.
Hou Z, Zhang Y.
Mol Cell. 2018; 70: 757-8.
Programmable RNA Cleavage and Recognition by a Natural CRISPR-Cas9 System from Neisseria meningitidis.
Rousseau BA, Hou Z, Gramelspacher MJ, Zhang Y.
Mol Cell. 2018; 69: 906-914.
The CRISPR-Cas9 system in Neisseria spp.
Pathog Dis. 2017; 75: ftx036.
Naturally Occurring Off-Switches for CRISPR-Cas9.
Pawluk A, Amrani N, Zhang Y, Garcia B, Hidalgo-Reyes Y, Lee J, Edraki A, Shah M, Sontheimer EJ, Maxwell KL, Davidson AR.
Cell. 2016; 167: 1829-38.
DNase H Activity of Neisseria meningitidis Cas9.
Zhang Y, Rajan R, Seifert HS, Mondragón A, Sontheimer EJ.
Mol Cell. 2015; 60: 242-55.
Cascading into focus.
Zhang Y, Sontheimer EJ.
Science. 2014; 345: 1452-3.
Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis.
Hou Z, Zhang Y, Propson NE, Howden SE, Chu LF, Sontheimer EJ, Thomson JA.
Proc Natl Acad Sci USA. 2013; 110: 15644-9.
Processing-independent CRISPR RNAs limit natural transformation in Neisseria meningitidis.
Zhang Y, Heidrich N, Ampattu BJ, Gunderson CW, Seifert HS, Schoen C, Vogel J, Sontheimer EJ.
Mol Cell. 2013; 50: 488-503
For a list of publications from Pubmed, click HERE