My laboratory has a major focus on GPCR-mediated signal transduction and uses a combination of biochemical, cell biology and in vivo methods to understand the signaling pathways regulated downstream of GPCRs. We have made significant contributions to GPCR dependent regulation of phosphoinositide signaling at both biochemical and signaling levels. We have identified novel signaling mechanisms regulated by both Gbeta and Gbeta/gamma subunits of G proteins. We have developed a pharmacological approach for targeting G protein beta/gamma subunits as a strategy for modulating opioid receptor biology, and treatment of cardiac hypertrophy and inflammation. Very recent work has focused on proteomic methods for identification of signaling networks driven downstream of activation of specific G protein signaling pathways.