My lab develops computer models of cellular networks – spanning metabolism, gene regulation and signaling, to understand how they interact with each other, and how these networks break down in disease. The computational approaches that we have developed (PROM, ASTRIX and GEMINI) perform complementary functions in reconstruction and modeling of biological networks. These methods integrate fundamental principles from biochemistry (thermodynamics, stoichiometry), engineering (such as mass and energy balance) and biophysics (probabilistic modeling) to model metabolic regulation. We are applying these systems approaches to tackle infectious diseases like Tuberculosis by understanding pathogen metabolism. We are also using these models to understand the interplay of metabolic pathways and transcriptional regulators in complex mammalian systems such as stem cells and immune cells.