Areas of Interest
We have developed four techniques based on bromouridine labeling of nascent RNA that allow us to estimate both the rate of transcription (Bru-seq) and the rate of degradation and splicing kinetics (BruChase-seq) of any RNA. In addition, the use of UV light to introduce random transcription-blocking lesions allowing us to map transcription start sites and active enhancer elements genome-wide (BruUV-seq). Finally, BruDRB-seq allows for the assessment of transcription elongation rates genome-wide. We have gotten funding from NIHGR and we participate in the ENCODE consortium to further develop the Bru-seq technologies.