Areas of Interest
The Uhler laboratory studies the role of protein phosphorylation in the nervous system. Historically, the research has focused on the cAMP-dependent (PKA) and cGMP-dependent (PKG) protein kinases. These kinases phosphorylate a large number of neuronal substrates including receptors , ion channels and transcription factors to regulate the function of these proteins. During the characterization of transcriptional regulation by PKA and PKG, our laboratory developed the Surface Transfection and Expression Protocol (STEP) method for solid phase transfection and we have used it to identify novel regulatory sequences. More recently, we have collaborated with the Turner laboratory to develop lines of P19 embryonic carcinoma cells which undergo neuronal differentiation after induction of neurogenic bHLH proteins such as Ascl1. We are currently using these cell lines to characterize the role of PKA phosphorylation during neuronal differentiation.