MDRC Molecular Genetics Core

RRID: SCR_015119

The Molecular Genetics Core provides services related to the application of genetic methodologies to research related to diabetes. 

Our services are currently run through MiCORESMiCORES is an online core management system from Agilent Technologies and iLab Solutions designed to streamline the process of requesting and billing for core services.

Already a MiCORES user? Go to our core page, sign in using your umich credentials and navigate to the “request services” page.

Not yet a MiCORES user? To get started you must register for an account. Once you are registered, go to our core page, sign in using your umich credentials and navigate to the “request services” page.

Generation of targeted genetic rodent models, using CRISPR/Cas9 system in mouse or rat embryos:
  • Gene edited alleles
  • Knockout alleles
  • loxP-flanked alleles
  • Knock-in alleles (gene insertions, e.g., cre and Flp recombinases, rtTA (“TET-on”), GFP)
  • Cost includes design, construct assembly, embryo injection costs, and screening of potential founders.
  • Application of CRISPR-Cas9 technology in specific genetic backgrounds is available on request, at extra cost.
Generation of specialty viral vectors:
  • Constitutive or cre-inducible AAVs expressing transgenes of interest
  • Cost includes design and construct assembly. Viral production costs are paid directly to the Viral Vector Core by user. ALL costs may be waived for reagents likely to be of general interest, providing that PI is willing to allow distribution to other MDRC members following initial testing.
Specialty viral reagents:
  • Defective pseudotyped rabies viruses for tract-tracing, including AAV helper viruses
  • Under development: AAV-driven CRISPR/Cas9 reagents for gene ablation in animals

Cost of Services

Rates Effective 10/1/2020 - 10/1/2021

* non-UM customers please contact David Olson for quote

Knock-In Rate
$10,242 per test
 
Knock-Out/Edit Rate
$8,882 per test
 
Flox Rate
$10,858 per test
Knock-In Rate
$4,982 per test
 
$4,582 per test
 
$4,345 per test
 
$3,013 per test
 
$1,011 per test
 
Lab Tech Services
$32 per hour

Service Request Form

* External Customers, please download, complete, and return the signed Service Agreement, along with the Service Request form and return to David Olson.

Key Personnel

David P. Olson, MD, PhD

Core Director

Qing Zhu, MD

Research Lab Specialist Associate

Thomas Lanigan, PhD

Co-Investigator

Huira Kopera, PhD

Vector Core Assistant Director

Dennis Larkin, BS

Research Laboratory Specialist

Contact Us

David P. Olson, MD, PhD

Brehm Tower Room 6442

Remember to cite the Michigan Diabetes Research Center (MDRC) in publications as follows:
“The project described was supported by Grant Number P30DK020572 (MDRC) from the National Institute of Diabetes and Digestive and Kidney Diseases”