This Cellular Physiology Laboratory provides MDRC investigators access to custom, specialized optogenetic and electrophysiological techniques and expertise. The services provided are tailored to the needs of individual MDRC investigators and include interrogation of neural circuits that control energy and glucose homeostasis.
- consultation to develop experimental design
- consultation and training for data analysis and interpretation
- optogenetic and/or electrophysiologic experiments for the generation of pilot data and/or small project collaboration, types of experiments include ex vivo patch clamp recordings from a variety of models including brain slice preparations, pancreatic islets and cell culture models.
- ex vivo live-cell calcium imaging in conjunction with the Imaging Laboratory
Use of the Optogenetic and Electrophysiology laboratory begins with consultation with Dr. Goforth for discussion of goals and feasibility. Dr. Goforth will then work with the MDRC investigator to devise an experimental plan and generate electrophysiology data. Extended studies will require training of laboratory personal by Dr. Goforth in the laboratory of the MDRC investigator.
The optogenetics/electrophysiology laboratory possesses an Olympus based fluorescence microscopy system mounted on a motorized translator and equipped with the necessary components for electrophysiologic experiments, including; two Sutter instruments MP-285 micromanipulators mounted on a Burleigh Gibraltar stage, a multiClamp 700B patch-clamp amplifier and Axon Digidata 1550 Data Acquisition system, a temperature control and perfusion system, and a Leica VT 1200S vibratome for brain slice preparation. The system also includes an LED-based optical illumination system for activation of genetically targeted light-activated opsins for remote control of cellular activity.
Remember to cite the Michigan Diabetes Research Center (MDRC) in publications as follows:
“The project described was supported by Grant Number P30DK020572 (MDRC) from the National Institute of Diabetes and Digestive and Kidney Diseases”