MICPC: In Situ Hybridization Lab

hybrydization

The in situ hybridization (ISH) core provides several methods of localization and quantification of RNA via the use of riboprobes. Different detection methods are available according to riboprobes labeling:

  1. Radioisotopes: autoradiographic film and emulsion
  2. Fluorescence: RNAprobe using RNAscope technology *

The core provides the following services:

  1. Consultation
  2. cDNA production
  3. Probe labeling by in vitro transcription
  4. Tissue processing
  5. Complete ISH procedure (hybridization and signal detection)
  6. Dual label ISH / immunohistochemistry
  7. Data analysis
  8. Training

Cost of Services

$207 per ISHH assay

$40 per ISHH assay

$556 per ISHH assay

$1,341 per ISHH assay

$302 per ISHH assay

Rates effective through 9/30/2020

The recharge for the above descriptions, measurements, and rates applies to all internal users of this service.

Service Agreement/Application

Remember to cite the Michigan Diabetes Research Center (MDRC) in publications as follows:
“The project described was supported by Grant Number P30DK020572 (MDRC) from the National Institute of Diabetes and Digestive and Kidney Diseases”