MICPC: In Situ Hybridization Lab

The in situ hybridization (ISH) lab provides several methods for localization and quantification of RNAs in tissue sections. Different detection methods are available according to probes labeling:
1.    Radioisotopes (35S and 33P): autoradiographic film and emulsion
2.    Fluorescence: RNAscope® technology
 
The core provides the following services:
1.    Consultation
2.    cDNA production
3.    Probe labeling by in vitro transcription
4.    Tissue processing
5.    Complete ISH procedure (hybridization and signal detection)
6.    Dual label ISH / immunohistochemistry (IHC)
7.    Single, dual or triple fluorescence labeling (RNAscope)
8.    Support with data analysis
9.    Training
 
Rates Effective 4/1/2021 - 4/1/2022

Cost of Services

RNA Scope
$3,022 per ISH assay - 3 probes, 20 slides*
 
Antibodies (for dual labeling)
$40 per ISH assay
 
Single ISH 35S
$800 per ISHH assay - 1 or 2 probes, 24 to 72 slides depending on the probes
 
Single ISH 33P
$1,775 per ISH assay - 1 or 2 probes, 24 to 72 slides depending on the probes
 
ISH Tissue Processing (sectioning in freezing microtome or cryostat)
$302 per ISH assay - 4 tissue blocks
 

*Contact Emily Henson (henson@umich.edu) for clarification or if changes in protocol are necessary.

 

The recharge for the above descriptions, measurements, and rates applies to all internal users of this service.

Remember to cite the Michigan Diabetes Research Center (MDRC) in publications as follows:
“The project described was supported by Grant Number P30DK020572 (MDRC) from the National Institute of Diabetes and Digestive and Kidney Diseases”