The in situ hybridization (ISH) core provides several methods of localization and quantification of RNA via the use of riboprobes. Different detection methods are available according to riboprobes labeling:
- Radioisotopes: autoradiographic film and emulsion
- Fluorescence: RNAprobe using RNAscope technology *
The core provides the following services:
- Consultation
- cDNA production
- Probe labeling by in vitro transcription
- Tissue processing
- Complete ISH procedure (hybridization and signal detection)
- Dual label ISH / immunohistochemistry
- Data analysis
- Training
