MICPC: In Situ Hybridization Lab

hybrydization

The in situ hybridization (ISH) core provides several methods of localization and quantification of RNA via the use of riboprobes. Different detection methods are available according to riboprobes labeling:

  1. Radioisotopes: autoradiographic film and emulsion
  2. Fluorescence: RNAprobe using RNAscope technology *

The core provides the following services:

  1. Consultation
  2. cDNA production
  3. Probe labeling by in vitro transcription
  4. Tissue processing
  5. Complete ISH procedure (hybridization and signal detection)
  6. Dual label ISH / immunohistochemistry
  7. Data analysis
  8. Training

 

Rates Effective 5/1/2020 - 5/1/2021

Cost of Services

RNA Scope
$207 per ISHH assay
 
Antibodies (for Double Label)
$40 per ISHH assay
 
Single ISHH 35S
$556 per ISHH assay
 
Single ISHH 33P
$1,341 per ISHH assay
 
Single ISHH Sample Sectioning/Mounting
$302 per ISHH assay

The recharge for the above descriptions, measurements, and rates applies to all internal users of this service.

Service Agreement/Application

Service Agreement/Application

Remember to cite the Michigan Diabetes Research Center (MDRC) in publications as follows:
“The project described was supported by Grant Number P30DK020572 (MDRC) from the National Institute of Diabetes and Digestive and Kidney Diseases”