Research Cores

Protein Localization, Identification and Folding Core

The Specific Aims of the Protein Localization, Identification and Folding (PLIF) core are as follows:

  • Provide state of the art protein localization and identification facilities. Core programs include: Imaging, Proteomics and Protein Folding Diseases Initiative resources.
  • Ensure delivery of high quality services and products and provide technical oversight of all PLIF services.
  • Train and educate members, associate members and pilot feasibility recipients in the application and use of protein identification techniques for the study of digestive and liver diseases.

For more information on the Protein Localization, Identification, and Folding Core, click here

In Vivo Animal and Human Studies Core

The Specific Aims of the In Vivo Animal and Human Studies (IVAHS) core are as follows:

  • To provide a variety of acute and chronic animal models, as well as state-of-the-art in vivo technologies to enhance rapid translation of basic discoveries to physiological and clinical applications;
  • To develop and maintain an organoid and enteroid program in which these preparations grown from mucosa biopsies and induced pluripotent stem cells (iPSCs) may be used to study epithelial cell biology and provide therapeutic targets for clinical intervention;
  • To support a clinical study program that will provide consultation and technical support to Center investigators on design and statistical data analysis; and
  • To provide a user friendly, one-step service to access digestive disease-related biospecimens for Center members. 

For more information on the In Vivo Animal and Human Studies core, click here.

Molecular Biology Core

The Specific Aims of the Molecular Biology (MB) core include:

  • To execute state of the art gene editing, gene transfer and gene profiling techniques in line with evolving member research needs.
  • To support highly trained personnel in the application of genetic technology, organized around four Core Programs: Transgenic Rodent, Genome Editing, Viral Vector and Integrated Genome Analysis.
  • To ensure delivery of high quality services and products and provide technical oversight of all Molecular Core services.
  • To train and educate members, associate members and pilot feasibility recipients in the application and use of molecular techniques for the study of digestive and liver diseases.

For more information on the Molecular Biology Core, click here.

Microbiome and Metabolomics Core

The Specific Aims for the UMCGR Microbiome and Metabolomics (MM) core include:

  • To provide the ability to retrieve nucleic acid, including genomic DNA and RNA, from human and animal tissue. This nucleic acid will be used for 16S, metagenomic and metatranscriptomic analysis for microbial communities. This material can also be exploited for expression analysis from host cells.
  • To facilitate the molecular analysis of the structure and function of complex microbial communities. The techniques for retrieval of small subunit rRNA-encoding gene sequence information will be available via the high-throughput generation of 16S variable region amplicon sequencing. Assistance with metagenomic sequence analysis using massively-parallel DNA sequencing will also be available. The generation and analysis of microbial genomes will be facilitated by the Core.
  • To provide expertise in germ free mouse technology. The ability to re-derive various mouse strains in the germ free state and to selectively colonize with specific microbes will be available in this Core.  Investigators will be able to maintain strains in germ-free or gnotobiotic states and also perform experimental procedures on these animals.
  • To provide access to metabolomic analysis of human and animal samples. Through the Michigan Metabolomics and Obesity Center’s metabolomic Core, members of the UMCGR will have access to state-of-the-art methods for targeted and untargeted measurement of host and microbial-derived small molecules to assess function of the microbiome.

For more information on the Microbiome and Metabolomics Core, click here.