Environmental cues can guide behavior adaptively but can also trigger maladaptive responses, such as relapse in individuals with substance use disorder (SUD). Some individuals are more prone to attributing incentive value to reward-associated cues, often due to deficits in inhibitory control. This study investigates the role of the glucocorticoid receptor (GR) in inhibitory control using transgenic Sprague-Dawley rats with selective GR knockdown in the prelimbic cortex (PrL) to nucleus accumbens core (NAcC) pathway. Results showed that rats with GR knockdown were more likely to attribute incentive salience to reward cues (sign-track). These findings suggest GR as a neuromolecular mechanism of inhibitory control within the corticostriatal pathway.