The Anumonwo laboratory studies the properties of an ion channel, which depends on its three-dimensional structure as well as on the interactions of the channel protein with other (accessory) proteins in specific micro-domains of the cell. A number of cardiac rhythm disturbances have been associated with mutant ion channel proteins, accessory proteins to the ion channels, or the improper interactions between the two proteins. Research in our laboratory focuses on understanding the molecular interactions of cardiac ion channel proteins under normal and pathophysiologial conditions. We use a combination of electrophysiological, biochemical and molecular biological techniques to carry out these investigations.
The Beard and Carlson lab (cooperating with Brian Carlson) is focused on systems engineering approaches for understanding the biophysical and biochemical operation of physiological systems. Dan Beard is the Director of the Virtual Physiological Rat (VPR) project, previously supported as an NIH National Center for Systems Biology, working to analyze, interpret, simulate, and ultimately predict physiological function in health and disease. The scope of topics in the lab cover integrated experimental and computational projects spanning the scales of subcellular to whole organism function and include: (1) Cardiac energy metabolism. (2) Cardiovascular system dynamics. (3) Regulation of coronary blood flow. (4) Stem cell derived cardiomyocytes.
Our research focuses on mechanisms of wave propagation and fibrillation using a combination of experimental, clinical, and numerical approaches with the aim of better understanding of acute and chronic atrial fibrillation as well as ventricular fibrillation. Current projects in Dr. Berenfeld's laboratory include:1. Dynamics of Impulse Propagation and Reentrant Activity.2. Mechanisms of Atrial Fibrillation. 3. Mapping of Cardiac Fibrillation. 4. Biophysical Mechanisms in Two Inherited Cardiac Diseases.
Research Associate Professor, Molecular & Integrative Physiology
The Beard and Carlson lab (cooperating with Daniel Beard) is focused on systems engineering approaches for understanding the biophysical and biochemical operation of physiological systems. Dan Beard is the Director of the Virtual Physiological Rat (VPR) project, previously supported as an NIH National Center for Systems Biology, working to analyze, interpret, simulate, and ultimately predict physiological function in health and disease. The scope of topics in the lab cover integrated experimental and computational projects spanning the scales of subcellular to whole organism function and include: (1) Cardiac energy metabolism. (2) Cardiovascular system dynamics. (3) Regulation of coronary blood flow. (4) Stem cell derived cardiomyocytes.
The long-term goal of Dr. Chen's laboratory is to stimulate bench-to-bedside research that sheds light on molecular mechanisms underlying the development and progression of diabetes-induced cardiovascular diseases (CVD). Discoveries from innovative and multi-disciplinary projects will reveal novel and effective intervention strategies to prevent and treat diabetes and CVD. In the past 15 years, Dr. Chen's laboratory has made a series of significant contributions to our understanding of the role of PPARgamma activation as a determinant of vascular cell gene expression and cellular function and has been among the first to begin to define the role of PPARdelta activation in the cardiovascular system. In addition, the discovery of the high affinity physiological PPARgamma ligands, nitroalkene derivatives of linoleic acid (LNO2) and oleic acid (OA-NO2), advances our understanding of endogenous PPARgamma modulation and provides novel therapeutic strategies for treating diabetes and CVD.
The major focus of the laboratory is to determine the impact of various genetic alterations on atherosclerosis and arterial thrombosis using in vivo mouse models. We have developed models of vascular injury in the setting of atherosclerosis that allow us to identify the impact of many genes and conditions on atherothrombosis. We have recently used these mouse models to study links between obesity, diabetes and vascular endpoints. We have demonstrated that factors produced by adipocytes are capable of directly affecting atherosclerosis and arterial thrombosis. In addition, while surveying factors in the plasma that are increased in vascular inflammatory conditions, including diabetes and atherosclerosis, we have identified factors that are consistently and markedly elevated. Importantly, we have now shown that these factors serve as highly informative biomarkers since they represent specific interactions between leukocytes and endothelial cells. Additional experiments designed to determine their role in vascular and adipose inflammation are underway. To address the broader role of adipose tissue inflammation in vascular disease, we have recently developed a model of visceral fat inflammation and demonstrated that visceral, but not subcutaneous fat inflammation, is sufficient to accelerate atherosclerosis – in the absence of diabetes. Efforts are ongoing to identify the specific proatherogenic factors that are released from inflammatory visceral fat.
James V Neel Distinguished University Professor, Internal Medicine & Human Genetics
Precise control of the blood-clotting system is essential for maintenance of the circulation in all higher animals. Deficient function of this system can lead to fatal bleeding following even a minor injury, whereas overactivity of this system can produce unwanted blood clots, resulting in blockages to critical blood vessels, as occurs in such diseases as heart attack and stroke. We study the molecular genetics of blood clotting, specifically von Willebrand factor, coagulation factor V and plasminogen activation.
The Isom laboratory focuses on understanding the molecular composition of individual sodium channel signaling complexes in excitable cells. We are testing the hypothesis that studying the conducting and non-conducting functions of the sodium channel beta subunits may yield important insights into the molecular basis of inherited disease. Part of our studies involve disruption of sodium channel signaling complexes in vivo and testing the consequences of such disruption on paroxysmal diseases such as cardiac arrhythmia and epilepsy. In addition, because sodium channel beta subunits can function as cell adhesion molecules in the absence of the ion conducting pore, we are studying whether mutations in beta subunit genes may result in defects in axon guidance or cell-cell communication. Our studies have significant clinical implications since it is already known that mutations in ion channels and their auxiliary subunits can lead to neurological or cardiovascular diseases.
The Michele laboratory focuses on the mechanisms of muscular dystrophy and cardiomyopathies associated with mutations in the transmembrane dystrophin-glycoprotein complex and abnormal glycosylation of the central protein in this complex, dystroglycan. The cellular mechanism of dystroglycan modification, and the resulting pathways leading to muscular dystrophy and cardiomyopathy are currently unclear. Our laboratory is currently exploring these mechanisms using spontaneous mutant, traditional and conditional targeted mouse models in vivo, and studying the effects on skeletal muscle function and cardiac myocyte biology in vitro.
Professor, Molecular & Integrative Physiology
Professor, Internal Medicine, Metabolism, Endocrinology, and Diabetes (MEND)
The Mortensen laboratory focuses on three areas: Diabetes, Obesity, and Cardiovascular Disease Signaling. The major goal of his laboratory has been to understand basic mechanisms in the consequences and treatment of obesity, diabetes and related cardiovascular disease. Our group focuses on the role of PPAR (peroxisome proliferators-activate receptor) transcription factors in adipose tissue formation, cell growth and cardiovascular disease (vascular and cardiac dysfunction in diabetes). Our studies with PPARs utilize a broad range of methodologies including transgenic and knockout animals, and physiologic telemetry measurements (blood pressure, EKG, temperature).
The Pinsky laboratory aims to elucidate the mechanisms by which blood vessels modulate their phenotype following periods of interrupted blood flow. Ultimately, the goals of our laboratory are to develop new insights into endogenous mechanisms of ischemic vascular injury and protection, in order to develop new therapeutic strategies targeted at the intersection of thrombotic, fibrinolytic, and inflammatory axes.
Professor, Molecular & Integrative Physiology
Professor, Internal Medicine, Division of Metabolism, Endocrinology & Diabetes
The Qi laboratory at the Brehm Tower integrates cellular, biochemical, immunological and physiological approaches to understand human health and disease. One of the laboratory’s main interests is to understand the pathogenesis of hyperlipidemia, with a strong focus on the intracellular trafficking of a key protein lipoprotein lipase (LPL). Our recent studies have identified a novel regulatory mechanism underlying LPL trafficking from the endoplasmic reticulum (ER) to extracellular space. Additional lines of research include studies of the role of ER homeostasis in various cell types in the context of obesity and diabetes, and the role of inflammation in pancreatic beta cell regeneration in diabetes.
The Runge lab has an active basic and translational science research program in the areas of atherosclerosis and vascular biology. We focus on two major goals: (1) developing novel diagnostic approaches for the early identification of coronary heart disease risk, and (2) understanding the role of oxidative stress and oxidative signaling in atherosclerosis to determine whether these pathways may offer targets for therapeutic intervention, specifically to inhibit plaque rupture and to retard myocardial infarction and stroke. We use cell culture and genetically engineered mouse models to study genes and signaling pathways critical for atherogenesis. These studies focus on understanding basic mechanisms with the goals of developing new diagnostic and therapeutic approaches with clinical applicability. We also are involved in collaborative studies on human atherosclerosis where we are currently studying novel molecular diagnostic tools in large clinical databases.
The Russell Laboratory examines the genetic determinants of heart development and the pathogenesis of human congenital heart defects. Using a zebrafish model, we are characterizing novel signaling pathways determined to be involved in cardiac outflow tract development based on the identification of genetic mutations in human patients with tetralogy of Fallot and hypoplastic left heart syndrome. In addition, we are continuing to search for additional novel disease genes using massively parallel sequencing of human patient samples.
Henry and Mala Dorfman Family Professor of Pediatric Hematology/Oncology
In the Shavit laboratory we perform “clinically directed basic research” in the field of hemostatic and thrombotic disorders. Patients with deficiencies of particular blood coagulation factors are often labeled with bleeding or clotting disorders, yet often have no phenotype. On the other hand there are patients with phenotypes out of proportion to their laboratory clotting factor profile. Thus we are often unable to predict an individual patient's risk with any useful degree of accuracy. Our goal is the identification of genes that modify blood clotting factors and their phenotypic expression. Knowledge of such modifier genes will improve diagnosis and classification of blood coagulation disorders, identify potential targets for therapy, and further our understanding of the underlying biology of hemostasis and thrombosis. In order to achieve these goals, we are developing zebrafish models of human blood clotting disorders.
Associate Professor, Cardiac Surgery
Associate Professor, Molecular & Integrative Physiology
The Westfall laboratory focuses on understanding myofilament function and its modulation by signaling cascades during health and disease. Ongoing studies are focused on 1) understanding the role of the third troponin I cluster in modulating contractile function, 2) evaluating whether there is an additive or synergistic effect of the 3 clusters on relaxation, 3) determining whether mutations within the regulatory portions of troponin I influence the phosphorylation response, and 4) investigating the role of these troponin I phosphorylation sites on contractile function under pathophysiological conditions and using gene transfer into myocytes from explanted failing human hearts to determine whether phosphomimetic troponin I mutants improve contractile performance.