Research Techniques

  • Animal models of cerebral ischemia, intracerebral hemorrhage, brain edema, peripheral nerve and spinal cord injury
  • Animal models of preconditioning
  • Cell culture (brain endothelial cells, neurons, astrocytes, choroid plexus epithelial cells)
  • Immunohistochemistry, Western and Northern blots, polymerase chain reaction
  • Measurement of cerebral blood flow with radiotracers or laser Doppler flowmetry
  • Mechanical implants for spinal cord regeneration
  • Nerve and spinal cord injury
  • Radioisotopes to measure blood-brain barrier permeability
  • Spinal cord stimulation
  • Ventriculo-cisternal perfusion

Equipment

  • Biophysical recorders
  • Blood gas analyzer
  • Cell harvester intravital video microscope
  • Computerized image analysis
  • Cryostat
  • Flame photometer
  • H2-clearance blood flow
  • Hydrogen blood flow electrodes
  • Inverted phase microscope (with Hoffman optics)
  • Laser doppler flowmeter
  • Liquid scintillation counter
  • Microelectrode amplifiers
  • Microelectrode puller
  • Microtiter plate washer
  • Operating microscopes
  • Operating room
  • Reverse-phase high-pressure liquid chromatography
  • Stereotactic frames
  • Thermal cycler-molecular biology equipment
  • Tissue culture hood and incubator
  • Ventilators