Morphology and Imaging Module

Director: Philip J. Gage, Ph.D.
Staffed by: Brad Nelson, Manager/Technician
Email: bbnelson@med.umich.edu
Phone: 734-615-7991
Fax: 734-936-3815

The Morphology and Imaging Module is a service and resource module for processing ocular and brain tissues for light microscopy, immunocytochemistry and in situ hybridization. Specimens can also be fixed for Electron Microscopy to be performed at other facilities. Training in tissue processing, microtomy, staining, and immunohistological techniques are also provided. The secondary function of the Module is to aid imaging of research results for analysis, presentation, and/or publication. Two computers are dedicated to producing publication-quality images and text from data produced by the Module Computer training in image production is also provided.

Submit a work order.

Module Instrumentation

Some pieces of equipment must be reserved for use. Reserve Equipment here.

Morphology

  • Leica CM3050 Cryostat
  • Lecia CM3050S disposable blade cryostat
  • Reichert steel knife sharpener 
  • Leitz rotary microtome for plastic sectioning
  • Tissue-Tek Paraffin processing and embedding station
  • Access to Shandon AS 325 paraffin microtome
  • Shandon Tissue Processor Embedding Station
  • Standard light level biological stains and staining setup available

Imaging

  • Research-quality Leica DM 6000 microscope with 2X-100X objectives, differential interference contrast optics (DIC), and transmitted and epi-fluorescence light sources. The microscope is fitted with duel digital cameras (greyscale for fluorescence, color for high resolution color stain) and interfaced with an HP computer with Leica’s LAS-X for image capture and analysis.
  • Epson Expression 1680 Flatbed scanner with dedicated G5 iMac running Adobe Creative suite (Adobe Photoshop, Illustrator, In-Design with PageMaker plug-in, Acrobat and Go-Live). Also Microsoft Office X, and OCR software
  • Nikon Super Coolscan 5000ED slide scanner, also associated with a G5 iMac
  • Access to the UM Microscopy and Image Analysis Laboratory (MIAL) in the Department of Cell and Developmental Biology for use of a BioRad or Zeiss laser scanning confocal microscope and a Philips transmission electron microscope
  • Olympus EX51 Microscope
  • Leica MetaMorph & LAS-AF Offline Imaging Workstation
  • Leica SP5 Confocal Microscope
  • Leica SPX5 2-Photon Laser-Scanning Confocal Microscope 
  • LMD7000 Laser Micro dissection Microscope
Morphology and Imaging module image