Ashley Munie

Ashley Munie

Immunology Program Graduate Student Candidate


Ashley began her graduate studies in 2017 at the University of Michigan before relocating to The Ohio State University with the Segal Lab. She remains a PhD candidate in the Immunology Graduate Program at UM. Prior to beginning graduate school, Ashley received her B.S in Biomedical Sciences from Murray State University in Murray, Kentucky. Her undergraduate work examined the role of autophagy in the context of C. elegans oogenesis under the advisement of Dr. Chris Trzepacz. Ashley also worked as a summer student during the summer of 2016 in the laboratory of Dr. Michael Barry studying the use of adenovirus vectors for oncolytic cancer immunotherapies. Since beginning her PhD studies, Ashley has been awarded the Bernard Maas Fellowship, the Rackham Pre-Candidacy Research Grant from Rackham Graduate School, and the Young Investigator Award from the American Association of Immunologists. Ashley currently serves as the graduate student representative for the Autumn Immunology Conference (AIC) general council. Ashley's work is currently funded by an NINDS F31 grant.

Research Interests

Ashley's thesis work focuses on interrogating immunoregulatory pathways in myeloid cells during Experimental Autoimmune Encephalomyelitis, a murine model of Multiple Sclerosis.


High-parameter flow cytometry, sc-RNAseq, bone marrow chimera techniques, antigen presentation assays, transwell assays, ELISA, PCR, qRT-PCR, IHC-C, MACS and FACS cell separation, CNS immune cell isolation,, bone marrow polarization techniques, primary murine cell culturing techniques, and mouse handling, oral gavage, peritoneal lavage, and injections (i.v., i.p., SQ).



Honors College Outstanding Research award, 2017, Murray State University

Lee Clark Outstanding Academic award, 2017, Murray State University

Outstanding Senior in Biology, 2017, Murray State University

Outstanding Undergraduate Teaching Assistant in Biology, 2017, Murray State University

McNair Scholar, 2015-2017 Murray State University

Summer Undergraduate Research Fellowship, 2016, Mayo Clinic

Office of Research and Creative Activity Research Fellowship, 2015-2016 Murray State University


Bernard Maas Fellowship, 2017, Rackham Graduate School, University of Michigan

Rackham Pre-Candidacy Research Grant, 2017, Rackham Graduate School, University of Michigan

Young Investigator Award, 2019, American Association of Immunologists

Neuroscience Day Best Basic Science Presentation, 2020, Neurology Department, Ohio State University


Munie, A., Duncker, P., Segal, B.M. GM-CSF promotes CNS autoimmunity via activation of the CCR1 chemokine pathway. Poster and Oral session presented at: Autumn Immunology Conference; 2018 November; Chicago, IL.

Munie, A., Segal, B.M. The Regulatory Role of TET2 in myeloid cells during EAE pathenogenesis. Poster and Oral session presented at: Autumn Immunology Conference; 2019 November; Chicago, IL.

Munie, A., Segal, B.M. The role of myeloid cell-specific ten eleven translocase 2 (TET2) in regulation of neuroinflammation. Poster and Oral session accepted for: AAI Immunology; 2020 May. Meeting cancelled due to COVID-19 pandemic. Abstract available online.



Mockus, T. E., Munie, A., Atkinson, J. R. & Segal, B. M. Encephalitogenic and Regulatory CD8 T Cells in Multiple Sclerosis and Its Animal Models. J. Immunol. 206, 3 (2021).

Hobbies and Extracurricular Activities

I like to spend my time outside of lab doing pottery, rock climbing, and playing ultimate frisbee. Of course, I also enjoy spending time with my dog Copernicus!