Areas of Interest
My focus is directed toward investigating the pathology of malfunctioning inhibitory cortical circuits associated with mental illnesses such as bipolar disorder and schizophrenia. To examine and isolate specific GABA-ergic interneuron subtypes in postmortem brain, I utilize gene expression analyses as well as implement neuroanatomical techniques including fluorescent in situ hybridization, brain transparency methods (e.g., CLARITY, iDISCO), and lightsheet microscopy.
Published Articles or Reviews
Medina AM*, Hagenauer MH*, Krolewski DM*, Hughes E, Forrester LC, Walsh DM, Waselus M, Richardson E, Turner CA, Sequeira A, Cartagena PM, Thompson RC, Vawter MP, Bunney BG, Myers RM, Barchas JD, Lee FS, Schatzberg AF, Bunney WE, Akil H, Watson SJ (2022) Neurotransmission-Related Gene Expression in the Frontal Pole (Brodmann Area 10) is Altered in Subjects with Bipolar Disorder and Schizophrenia. medRxiv 2022.06.03.22275600. * Equal first authors
Kumar V, Krolewski DM, Hebda-Bauer EK, Parsegian A, Martin B, Foltz M, Akil H, Watson SJ. (2021) Optimization and evaluation of fluorescence in situ hybridization chain reaction in cleared fresh-frozen brain tissues. Brain Struct Funct. Mar;226(2):481-499.
Krolewski DM, Kumar V, Martin B, Tomer R, Deisseroth K, Myers RM, Schatzberg AF, Lee FS, Barchas JD, Bunney WE, Akil H, Watson SJ Jr. (2018) Quantitative validation of immunofluorescence and lectin staining using reduced CLARITY acrylamide formulations. Brain Struct Funct. Mar;223(2):987-999.
Wei Q, Krolewski DM, Moore S, Kumar V, Li F, Martin B, Tomer R, Murphy GG, Deisseroth K, Watson SJ Jr, Akil H. (2018) Uneven balance of power between hypothalamic peptidergic neurons in the control of feeding. Proc Natl Acad Sci U S A. Oct 2;115(40): E9489-E9498.