Animal Modeling Core

The UM-SBDRC AMC supplies consultation for:

• Design and development of project-specific Genetically Engineered Mouse Models (GEMMs), including conventional, Cre-inducible, doxycycline-inducible, and conditional mutant mice.
• Production and validation of mouse models, including transgene construction, verification, genotyping, and GEMM production; screening; breeding, strain establishment, and validation.

The UM-SBDRC AMC supplies training in:

• In vivo manipulation, including transgene induction protocols, UV irradiation, induction of skin inflammation by exogenous agents, bleomycin-induced fibrosis, and orthotopic xenografts and allografts.
• GEMM phenotyping, including proper tissue collection and processing; morphologic, biochemical, and molecular characterization; cross-species validation.
• Establishment of GEMM-derived primary cultures and immortalized cell lines, taking into consideration key experimental variables including body site, gender, and age.